Everyone is welcome to join our QTHA Seminar presented by James Cook University, School of Public Health, Tropical Medicine and Rehabilitation Sciences
Friday 21st February 2014, 11am,
Dr Jennifer Wilson, Institute for Glycomics, Griffith University
"Structural adventures in unravelling the lifestyles of Gram negative bacteria."
James Cook University, Cairns A21.001 Teaching Annex; Videolinked to James Cook University, Townsville Bldg 9 MP AV Services 001 (DA 9001)
Dr Jennifer Wilson's talk will cover two topics:
Moraxella bovis is the Gram negative microorganism that causes infectious bovine keratoconjunctivitis (IBK or pink eye), a disease that causes severe keratoconjunctivitis, ocular rupture or blindness in cattle. Although carbohydrate structures such as lipopolysaccharide/lipooligosaccharide and capsule are known virulence factors for many microorganisms surprising little is known regarding the presence and composition of these structures for M. bovis. Structural elucidation of the oligosaccharide structure of the rough phenotype of Moraxella bovis strain Epp63 was determined using GC-MS, methylation analysis and NMR spectroscopy. The oligosaccharide is a branched structure containing 10 sugars in addition to KDO and contains some unusual features. Using glycosytransferase mutant bacteria we have also been able to identify the function of some of the glycosyltransferase enzymes important in LOS biosynthesis. Biological evaluation of these truncated LOS mutants has provided insight into the role of the OS.
Campylobacter jejuni is the leading cause of human gastroenteritis worldwide with over 500 million cases annually. Chemotaxis and motility have been identified as important virulence factors associated with C. jejuni colonisation. Group A transducer-like proteins (Tlps) are responsible for sensing the external environment for bacterial movement to or away from a chemical gradient or stimulus. In this study, we have demonstrated Cj1564 (Tlp3) to be a multi-ligand binding chemoreceptor and report direct evidence supporting the involvement of Cj1564 (Tlp3) in the chemotaxis signalling pathway via small molecule arrays, surface plasmon and nuclear magnetic resonance (SPR and NMR) as well as chemotaxis assays of wild type and isogenic mutant strains. Here we demonstrate the ability of Cj1564 to interact with the chemoattractants and chemorepellents. This is the first report characterising Cj1564 as a multi-ligand receptor for C. jejuni, and illustrates its involvement in the chemotaxis pathway and subsequent survival of this organism in the host.
Bio: Dr Jennifer Wilson is a Senior Lecturer and Research Leader at Griffith University Gold Coast Campus. After graduating in 1990 from Melbourne University in Inorganic Chemistry she was awarded a postdoctoral fellowship in 1990 (NOW-SON) to study with Prof Dr Jan Reedijk at Leiden University in the Netherlands. From 1991-2000, she worked at the Victorian College of Pharmacy, Monash University (now MIPS) firstly with Prof David Craik and then Prof Mark von Itzstein. From 1996 she managed the NMR facility at VCP. In 2000 she accepted a lectureship at Griffith University Gold Coast Campus where she is a member of the Institute for Glycomics and a member of the School of Medical Science in the Health Faculty. Dr Wilson has been on NHMRC panels (Development grant and CDA) continuously since 2003. Since 2008 she has been awarded $2,310K in NCG funding. She has >50 research articles including journal articles, invited book chapters and reviews, many in outstanding journals such as Plos Pathogen, JACS, J.Biol Chem, J.Mol. Biol and Brain, some with > 60 citations.